Journal: Immunity

Article Title: Notch4 signaling limits regulatory T-cell-mediated tissue repair and promotes severe lung inflammation in viral infections

doi: 10.1016/j.immuni.2021.04.002

Figure Lengend Snippet:

Article Snippet: AREG antibody , BiorByt , orb676838.

Techniques: Recombinant, Adjuvant, Enzyme-linked Immunosorbent Assay, Control, Blocking Assay, Mutagenesis, Software

Journal: Acta Biochimica et Biophysica Sinica

Article Title: HDAC11 in ovarian granulosa cells coordinates LH in the maturation of oocytes in Tan sheep

doi: 10.3724/abbs.2025036

Figure Lengend Snippet: Expressions and secretion of AREG, EREG and BTC in cultured granulosa cells of Tan sheep by HDAC11 inhibition (A) Expressions of AREG, EREG, BTC were verified by western blot analysis. (B) The quantification of the protein levels of panel (A). (C) Protein quantification of AREG, EREG and BTC at 24 h by ELISA. (D–F) The expression levels of AREG (D), EREG (E) and BTC (F) in cultured granulosa cells after HDAC11 inhibition. Scale bar = 200 μm. (G) The quantification of the fluorescence intensity of panel (D–F). All experiments were performed in triplicate and the data were presented as the mean ± SEM. ns, no significance, *P < 0.05, **P < 0.01.

Article Snippet: Subsequently, the membranes were incubated for 1 h at room temperature with 5% BSA, followed by an overnight incubation with the primary antibodies against AREG (PU595967S, 1:1000; ABmart, Shanghai, China), EREG (A16372, 1:1000; Abclonal, Wuhan, China), BTC (DF7038, 1:1000; Affinity), PCNA (10205-2-AP, 1:1000; Proteintech, Wuhan, China), H3K9ac (ab32129, 1:500; Abcam, Cambridge, UK), H3K27ac (ab52946, 1:2000; Abcam), H4K12ac (ab177793, 1:10,000; Abcam), H4K5ac (ab51997, 1:10,000, Abcam), GAPDH (10494-1-AP, 1:1000; Proteintech), acetylated-Lysine (9814, 1:1000; Cell Signaling, Danvers, USA), YAP1 (ab52771, 1:1000; Abcam), P53 (AF0879, 1:1000; Affinity), or histone H3 (AF863, 1:1000; Affinity) at 4°C.

Techniques: Cell Culture, Inhibition, Western Blot, Enzyme-linked Immunosorbent Assay, Expressing, Fluorescence